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Saudi Journal of Kidney Diseases and Transplantation
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ORIGINAL ARTICLE Table of Contents   
Year : 2007  |  Volume : 18  |  Issue : 4  |  Page : 523-531
Evaluation of the available anti-HCV antibody Detection Tests and RT-PCR assay in the Diagnosis of Hepatitis C Virus Infection


1 Consultant Internal Medicine and Nephrology, Al-Noor Specialist Hospital, Makkah, Saudi Arabia
2 Director of Laboratory Department, Al-Noor Specialist Hospital, Makkah, Saudi Arabia
3 Professor of Clinical Pathology, Al-Noor Specialist Hospital, Makkah, Saudi Arabia
4 General Surgery Consultant, Al-Noor Specialist Hospital, Makkah, Saudi Arabia
5 Consultant Diabetes, Al-Noor Specialist Hospital, Makkah, Saudi Arabia
6 Prof. Benha Faculty of Medicine, Al-Noor Specialist Hospital, Makkah, Saudi Arabia

Correspondence Address:
Zohair J Gazzaz
Consultant Diabetes, Health Research Center Director, Al-Noor Specialist Hospital, Makkah
Saudi Arabia
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PMID: 17951937

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The aim of the present work is to evaluate the commercially available antibody tests in the diagnosis of hepatitis C virus (HCV) infection by comparing their results with the RT-PCR test. The study included 316 serum samples from three groups: blood donors, patients on maintenance hemodialysis (HD) and patients infected with the human immunodeficiency virus (HIV). Samples were subjected to HCV-antibody detection by ELISA and RIBA tests and HCV­RNA detection by RT-PCR assay. The percentage of infectivity for blood donors was 18.9% by ELISA, 20.8% by RIBA and 23.6% by RT-PCR test. For patients on HD and those positive for HIV, the test positivity was respectively 59.3% and 5.3% by ELISA, 64% and 10.5% by RIBA and, 66.3% and 21% by PCR test. The percentage of false negativity of HCV-Ab by ELISA and RIBA when compared with RT-PCR test was 3.5 and 8.1% for samples blood donors, 17.1 and 25.7% for HD patients and 5.6 and 16.7% for HIV-infected samples, respectively. The false positivity of HCV-Ab by ELISA and RIBA, when compared with RT-PCR, was 5%, 3.9% and zero for blood donors, HD patients and HIV-HCV co-infected cases, respectively. While comparing ELISA with RT-PCR, the false positivity was 10%, 5.9% and zero respectively for blood donors, HD patients and HIV-HCV co-infected cases. Thus, it is very important to screen blood donors, HD patients and HIV-infected patients by using the RT-PCR for HCV-RNA to avoid false negative results.


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