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Saudi Journal of Kidney Diseases and Transplantation
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Year : 2008  |  Volume : 19  |  Issue : 3  |  Page : 378-383
Stabilizing Effects of Cool Dialysate Temperature on Hemodynamic Parameters in Diabetic Patients Undergoing Hemodialysis


1 Prevention of Metabolic Disorders Research Center, Research Institute for Endocrine Sciences, Shaheed Beheshti University of Medical Sciences, Tehran, Iran
2 Department of Hemodialysis, 15 Khordad Hospital, Shaheed Beheshti University of Medical Sciences, Tehran, Iran

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   Abstract 

To investigate the effect of cool dialysis on hemodynamic parameters and serum nitric oxide levels in diabetic patients, we studied 20 old (mean age 63.3 ± 7.5) chronic hemodialysis diabetics who were dialyzed twice, once using cool and once using standard (37 o C) temperature dialysate solution. During the study, all the dialysis conditions were maintained the same except coo­ling the dialysate from 37 o C to 35°C. Hemodynamic parameters including SBP, DBP, and HR were measured hourly. Oral temperature was measured before and after dialysis. Serum urea and nitric oxide metabolites were determined before and after hemodialysis. Systolic, diastolic, and mean arterial pressure decreased significantly during standard temperature compared to cool dialysis. Maximum decrease of systolic, diastolic, and mean arterial pressure was observed during the third hour of dia­lysis and the magnitude of decrease was 18, 17, and 14 percent for standard temperature and 6, 1, and 4 percent for cool dialysis, respectively. Heart rate did not differ significantly between the two study groups. Compared to the pre dialysis levels of serum nitric oxide metabolites, the post dialy­sis levels decreased significantly with cool and standard temperature dialysate (59 ± 5 vs. 37 ± 4, and 63 ± 7 vs. 41 ± 5, µmol/L respectively, P< 0.01). Cool dialysis could decrease episodes of hypotension and stabilized hemodynamic parameters in diabetic patients. Probably other mechanisms than in­creased serum nitric oxide levels may be involved in hemodialysis hypotension in this group of patients.

Keywords: Cool dialysis, Diabetes, Hemodialysis, Hemodynamic parameters, Nitric oxide

How to cite this article:
Ghasemi A, Shafiee M, Rowghani K. Stabilizing Effects of Cool Dialysate Temperature on Hemodynamic Parameters in Diabetic Patients Undergoing Hemodialysis. Saudi J Kidney Dis Transpl 2008;19:378-83

How to cite this URL:
Ghasemi A, Shafiee M, Rowghani K. Stabilizing Effects of Cool Dialysate Temperature on Hemodynamic Parameters in Diabetic Patients Undergoing Hemodialysis. Saudi J Kidney Dis Transpl [serial online] 2008 [cited 2019 Aug 17];19:378-83. Available from: http://www.sjkdt.org/text.asp?2008/19/3/378/40496

   Introduction Top


Hypotension is the most common acute complication of hemodialysis (HD). [1] Cool temperature dialysate is used for amelio­ration of symptomatic hypotension during HD. [2] Because this method is simple and cost effective, it has increased popularity in HD centers. [2] However, not all patients be­nefit from the cool dialysis, [3] and some HD complications such as cramps seem to be more frequent with cool (35 o C) than stan­dard (37 o C) temperature dialysate. [2]

Although cool dialysate is used to stabi­lize blood pressure during HD, some reports found that decreasing dialysate tempera­ture had no beneficial effect on mean arte­rial pressure in patients with stable blood pressure. [4] Without appropriate selection of patients for cool dialysis may result in effect not better than placebo. [5] Groups including women, patients over 55 years of age, pa­tients with low body surface area, and pa­tients with cardiovascular disease appear to benefit most from cool dialysis. [6]

Based on our knowledge, there is no re­port that exclusively investigated the effect of cool dialysis on diabetic HD patients who usually are at increased risk for hypo­tension during HD. [7] Over the years, the percentage of dialyzed diabetic patients in­creased tremendously. [7] Therefore, preven­ting hypotension episodes during HD may be beneficial in this group of patients. [8] In addition, previous studies have found that accumulation of nitric oxide is one of the major causes of hypotension during HD. [9]

The aim of our present study is to deter­mine the effect of cool dialysate temperature (i.e., 35°C) compared to standard dialysate (i.e., 37°C) on the hemodynamic stability in diabetic patients. We also measured serum nitric oxide metabolites pre and post dialy­sis to determine the role of nitric oxide in HD hypotension.


   Patients and Methods Top


We studied in a crossover design 20 (10 men and 10 women) old (63.3 ± 7.5 years) diabetic patients who were receiving 4­hour maintenance HD three times a week for a mean duration of 28 ± 4 months at the dialysis center of Shaheed Beheshti Univer­sity of Medical Sciences. Informed consent was obtained from all patients, and the pro­tocol was approved by the ethical commi­ttee of the university.

Each patient was dialyzed twice, once with standard temperature and once with cool dialysate. During the study, all the dialysis conditions were maintained the same except cooling the dialysate from 37 o C to 35°C. All patients were dialyzed using R5 capillary dialyzers and acetate dialysate produced by Behvarzan Pharmaceutical Company, Tehran, Iran (Na + 135, K+ 2, Ca 2+ 2.5, Mg 2+ 1.0, acetate 35, and Cl - 105.5 meq/L) at a flow rate of 250 ml/min. Systolic and dias­tolic blood pressures (SBP & DBP) and heart rate (HR) were measured every hour during dialysis. Mean arterial pressure (MAP) was computed as the diastolic blood pressure plus one third of the pulse pressure. Hypo­tension episode was defined as SBP less than 90 mm Hg, or a 25 % decrease from basal levels if baseline SBP was 90-100 mmHg. [2]

Oral temperature was measured before and immediately after dialysis. Serum urea concentration was measured pre and post dialysis by a commercial Kit (Man Co. Tehran, Iran). The equilibrated Kt/V(Kt/Veq) was determined using equation 1 as pre­viously reported. [4] Kt/Veq = (1-0.47/t) ×Kt/ Vsp + 0.02. The single pool Kt/V(Kt/Vsp) was calculated as: Kt/Vsp = -Ln (R-0.008 × t) + (4-3.5 × R) × UF/W , where R = urea post/urea pre; UF = ultrafiltration volume in Kg; T = treatment time in h; and W = body weight in kg. Urea reduction rate (URR), was calculated by formula: urea pre-urea post/urea pre × 100%.

Total nitrite and nitrate (NOx) levels mea­sured spectrophotometrically as previously reported by Miranda et al. [10] The principle of this assay is the reduction of nitrate by vanadium (III) combined with detection by the acidic Griess reaction. The sensitivity of this method is 0.5 µmol/L and is useful in a variety of fluids including serum.


   Statistical Analysis Top


Data were reported as mean ± SE ( stan­dard error). Statistical analysis was performed by SPSS program (Version 11.5). Paired sample t-test or two-way mixed between­within analysis of variance (ANOVA) were used to compare NOx levels and hemo­dynamic changes, respectively. McNemar test was used for comparison of hypotension episodes during cool and standard tempe­rature dialysis.


   Results Top


[Table - 1] reveals that the parameters of Kt/ Veq, ultrafiltration, urea reduction rate, and predialysis temperature were not signifi­cantly different between the study groups. Post dialysis temperature was significantly less in the cool dialysis than the standard temperature dialysis group as predicted (P<0.01). Patients dialyzed with cool dia­lysate experienced significantly fewer epi­sodes of hypotension than those dialyzed with standard temperature dialysate, 15.8% and 57.9%, respectively (P<0.05). Except for heart rate (HR), all other hemodynamic parameters including systolic, diastolic, and mean arterial pressures were significantly less in standard temperature compared to cool dialysis, (P<0.05). Maximum decrease in SBP, DBP, and MAP occurred at the third hour of dialysis and were 18, 17, and 14 % for standard temperature and 6, 1, and 4 % for cool dialysis, [Figure - 1].

Measurement of nitric oxide metabolites be­fore and after dialysis revealed that in both standard temperature and cool dialysis con­ditions serum NOx levels decreased signi­ficantly after dialysis (63 ± 7 vs. 41 ± 5 and 59 ± 5 vs.37 ± 4 µmol/L for standard tem­perature and cool dialysis respectively, P < 0.01). There was no significant difference between post dialysis NOx levels in both dialysis temperatures, [Figure - 2].


   Discussion Top


In this study we found that cool dialysis compared to standard temperature dialysis had beneficial effects for diabetic patients and caused more stability in hemodynamic parameters during HD without decrease in the HD efficiency. Although not restricted to diabetic patients this finding is in line with other reports that lowering the dia­lysis temperature decrease the number of hypotensive episodes in HD patients and has no effect on dialysis efficiency. [4],[11] How­ever, It should be mentioned that in this study, Kt/Veq ratio observed in both situa­tions was close to 1.0, which was less than the minimum standard level of 1.2 repor­ted by others. [4] The percentages of hypo­tensive episodes were relatively high in our study (57.9% and 15.8 % in standard tem­perature and cool dialysis, respectively). Prevalence between 20-50% of symptomatic hypotension in HD was reported elsewhere. [2] Patients with subnormal body temperatures gain the maximum benefit from cool dia­lysis and euthermic patients need not be exposed to 35°C dialysis as it may not have beneficial effect and may be unnece­ssarily uncomfortable. [12] Although we did not select patients based on predialysis body temperature, subsequent analysis showed that non of the subjects had predialysis sub­normal body temperature (i.e., < 36°C).

Nitric oxide is considered as a mediator of hypothermic dialysis [13] and accumulation of nitric oxide is reported as a cause of dia­lysis hypotension. [9],[14] In this study, serum NOx levels decreased significantly after dialysis in both standard temperature and cool dialysis but there was no significant difference between both groups, which is compatible with findings of Beerenhout et al, [15] who found higher production of NOx in diabetic patients on standard tempera­ture dialysis than on cool dialysis. The effect of cool temperature dialysis on nitric oxide production in diabetic patients needs more investigation. Nitrate is a normal constituent of human urine. [16] Renal clearance of nitrate is 20 ml/min which reflects substantial tu­bular reabsorption. [17] Nitrate accumulates in serum of patients between HD sessions and decreased serum levels after HD is due to its clearance with dialysis. Chronic renal failure is a state of NOx deficiency, but its plasma levels in these patients are higher than normal subjects. [18]

In conclusion, the results of this study suggest the use of simple and low cost me­thod such as decreasing dialysate tempera­ture to prevent dialysis hypotension in dia­betic patients and demonstrate that probab­ly other mechanisms than increased nitric oxide production may be responsible for dialysis hypotension in diabetic patients.


   Acknowledgements Top


This study was supported by a grant from Prevention of Metabolic Disorders Research Center, Research Institute for Endocrine Sciences, and 15 Khordad Hospital, Shaheed Beheshti University of Medical Sciences, Tehran, Iran. We would like to thank Mrs. M. Padyab for statistical helps.

 
   References Top

1.Bregman H, Daugirdas J, Ing T. Complications during hemodialysis. In: Daugirdas J, Blake P, Ing T (eds). Handbook of dialysis. Lippincott Williams & Wilkins, USA: 2001;148-68.  Back to cited text no. 1    
2.Marcen R, Quereda C, Orofino L, et al. Hemodialysis with low-temperature dialysate: A long-term experience. Nephron 1988;49 (1):29-32.  Back to cited text no. 2    
3.Maggiore Q. Isothermic dialysis for hypo­tension-prone patients. Semin Dial 2002; 15(3):187-90.  Back to cited text no. 3    
4.Ayoub A, Finlayson M. Effect of cool temperature dialysate on the quality and patients perception of haemodialysis. Nephrol Dial Transplant 2004;19(1):190-4.  Back to cited text no. 4    
5.Marcen R, Orofino L, Quereda C, Pascual J, Ortuno J. Effects of cool dialysate in dialysis-related symptoms. Nephron 1990; 54(4):356-7.  Back to cited text no. 5    
6.Orofino L, Marcen R, Quereda C, et al. Epidemiology of symptomatic hypotension in hemodialysis: Is cool dialysate beneficial for all patients? Am J Nephrol 1990;10(3): 177-80.  Back to cited text no. 6    
7.Cruz DN, Mahnensmith RL, Brickel HM, Perazella MA. Midodrine and cool dialy­sate are effective therapies for symptom­matic intradialytic hypotension. Am J Kidney Dis 1999;33(5):920-6.  Back to cited text no. 7    
8.Franssen CF, Dasselaar JJ, Sytsma P, Burgerhof JG, de Jong PE, Huisman RM. Automatic feedback control of relative blood volume changes during hemodialysis improves blood pressure stability during and after dialysis. Hemodial Int 2005;9(4): 383-92.  Back to cited text no. 8    
9.Dheenan S, Henrich WL. Preventing dialysis hypotension: A comparison of usual protective maneuvers. Kidney Int 2001;59 (3):1175-81.  Back to cited text no. 9    
10.Miranda KM, Espey MG, Wink DA. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 2001;5(1):62-71.  Back to cited text no. 10    
11.Yu AW, Ing TS, Zabaneh RI, Daugirdas JT. Effect of dialysate temperature on central hemodynamics and urea kinetics. Kidney Int 1995;48(1):237-43.  Back to cited text no. 11    
12.Fine A, Penner B. The protective effect of cool dialysate is dependent on patients' predialysis temperature. Am J Kidney Dis 1996;28(2):262-5.  Back to cited text no. 12    
13.Jamil KM, Yokoyama K, Takemoto F, Hara S, Yamada A. Low temperature hemo­dialysis prevents hypotensive episodes by reducing nitric oxide synthesis. Nephron 2000;84(3):284-6.  Back to cited text no. 13    
14.Yokokawa K, Mankus R, Saklayen MG, et al. Increased nitric oxide production in pa­tients with hypotension during hemodia­lysis. Ann Intern Med 1995;123(1):35-7.  Back to cited text no. 14    
15.Beerenhout CH, Noris M, Kooman JP, et al. Nitric oxide synthetic capacity in relation to dialysate temperature. Blood Purif 2004;22(2):203-9.  Back to cited text no. 15    
16.Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite and (15N) nitrate in biological fluids. Anal Biochem 1982; 126(1):131-8.  Back to cited text no. 16    
17.Wennmalm A, Benthin G, Edlund A, et al. Metabolism and excretion of nitric oxide in humans: An experimental and clinical study. Circ Res 1993;73(6):1121-7.  Back to cited text no. 17    
18.Blum M, Yachnin T, Wollman Y, et al. Low nitric oxide production in patients with chronic renal failure. Nephron 1998; 79(3):265-8.  Back to cited text no. 18    

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Correspondence Address:
Asghar Ghasemi
Research Institute for Endocrine Sciences, Shaheed Beheshti University of Medical Sciences, P.O. Box 19395-4763 Tehran
Iran
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PMID: 18445896

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This article has been cited by
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    Abstract
    Introduction
    Patients and Methods
    Statistical Analysis
    Results
    Discussion
    Acknowledgements
    References
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