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Saudi Journal of Kidney Diseases and Transplantation
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SPECIAL ARTICLE Table of Contents   
Year : 2009  |  Volume : 20  |  Issue : 4  |  Page : 662-665
Pronase-free B-cell flow-cytometry crossmatch


1 College of Medicine, King Saud bin Abdulaziz University for Health Sciences and Department of Pathology and Laboratory Medicine, King Abdulaziz Medical City, Riyadh, Saudi Arabia
2 Department of Pathology and Laboratory Medicine, King Abdulaziz Medical City, Riyadh, Saudi Arabia

Correspondence Address:
A H Hajeer
Dept. of Pathology and Laboratory Medicine, King Abdulaziz Medical City, P.O. Box 22490, Riyadh11426
Saudi Arabia
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PMID: 19587513

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Detection of anti-class II antibodies by panel response assay (PRA) and flow cross­match techniques carries an important value in terms of graft function. Even low levels of pre­formed alloantibodies to HLA class II antigens represent a risk of rejection. We present here a method for blocking non-specific flow crossmatch reactions using pooled, heat-inactivated rabbit serum. This method shows very low background and minimal non-specific reactions. In addition, it avoids the use pronase enzyme that can non-specifically digest different cell surface proteins.


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