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Saudi Journal of Kidney Diseases and Transplantation
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ORIGINAL ARTICLE Table of Contents   
Year : 2010  |  Volume : 21  |  Issue : 5  |  Page : 852-858
Oxidative stress in hemodialysis patients receiving intravenous iron therapy and the role of N-acetylcysteine in preventing oxidative stress


1 Department of Nephrology, Nizams Institute of Medical Sciences, Hyderabad, India
2 Department of Clinical Pharmacology, Nizams Institute of Medical Sciences, Hyderabad, India

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Date of Web Publication31-Aug-2010
 

   Abstract 

To determine the contribution of injectable iron administered to hemodialysis (HD) patients in causing oxidative stress and the beneficial effect of N-acetylcysteine (NAC) in re­ducing it, we studied in a prospective, double blinded, randomized controlled, cross over trial 14 adult HD patients who were randomized into two groups; one group received NAC in a dose of 600 mgs twice daily for 10 days prior to intravenous iron therapy and the other group received placebo. Both the groups were subjected to intravenous iron therapy, 100 mg of iron sucrose in 100 mL of normal saline given over a period of one hour. Blood samples for the markers of oxidative stress were taken before and after iron therapy. After the allowance of a week of wash out period for the effect of N-acetylcysteine we crossed over the patients to the opposite regimen. We measured the lipid peroxidation marker, malondiaaldehyde (MDA), to evaluate the oxidative stress and total anti-oxidant capacity (TAC) for the antioxidant level in addition to the highly sensitive C-reactive protein (HsCRP). Non-invasive assessment of endothelial dysfunction was measured by digital plethysmography before and after intravenous iron therapy. There was an increase of MDA (21.97 + 3.65% vs 7.06 + 3.65%) and highly sensitive C-reactive protein (HsCRP) (11.19 + 24.63% vs 13.19 + 7.7%) after iron administration both in the placebo and the NAC groups. NAC reduced the baseline acute systemic generation of oxidative stress when com­pared to placebo, which was statistically significant with MDA (12.76 + 4.4% vs 9.37 + 4.40%: P = 0.032) but not with HsCRP though there was a declining trend (2.85 + 22.75 % vs 8.93 + 5.19%: P = 0.112). Pre-treatment with NAC reduced the endothelial dysfunction when compared to placebo, but it was not statistically significant, except for reflection index (RI). We conclude that in our HD patients NAC reduced the oxidative stress before and after the administration of intravenous iron therapy in addition to the endothelial dysfunction induced by this treatment.

How to cite this article:
Swarnalatha G, Ram R, Neela P, Naidu M U, Dakshina Murty K V. Oxidative stress in hemodialysis patients receiving intravenous iron therapy and the role of N-acetylcysteine in preventing oxidative stress. Saudi J Kidney Dis Transpl 2010;21:852-8

How to cite this URL:
Swarnalatha G, Ram R, Neela P, Naidu M U, Dakshina Murty K V. Oxidative stress in hemodialysis patients receiving intravenous iron therapy and the role of N-acetylcysteine in preventing oxidative stress. Saudi J Kidney Dis Transpl [serial online] 2010 [cited 2019 Aug 20];21:852-8. Available from: http://www.sjkdt.org/text.asp?2010/21/5/852/68879

   Introduction Top


Oxidative stress is defined as a disruption of the equilibrium between the oxidants and the activity of antioxidant systems. The major source of oxidants is provided by the circulating po­lymorph nuclear (PMN) neutrophils and mono-cytes, when activated by uremic toxins or bio­incompatible dialysis membranes. [1],[2]

With respect to the markers of oxidative stress, plasma level of malondialdehyde (MDA), a byproduct of lipid peroxidation that reacts with thiobarbituric acid (TBA), TBA-reacting substances (TBARS), which is elevated in both dialyzed and nondialyzed CRF patients. [3],[4],[5] Ad­vanced oxidation protein products (AOPP) are a novel oxidative stress marker formed as a re­sult of oxidation of particularly sensitive amino acid residues, present at high levels in the plas­ma of uremic patients. [6] Dityrosine, which is generated from covalent binding links between two tyrosine residues, is a selective marker of protein attack. [7],[8],[9] Formation of carbonyl residues represents another early maker of protein oxi­dation. [10],[16] It is also established that thiols such as homocyteine, are able to generate reactive oxygen species (ROS). [11],[12]

Physiologic protection against oxidants in­volves both enzymatic antioxidant systems that include dismutase, catalase, and glutathione peroxidase and antioxidant molecules without enzymatic activities, which are also called sca­vengers and comprise, such as glutathione di­sulphide-containing tripeptide, present in all cell types and capable of scavenging H 2 O 2 , O 2 , and OH and chlorinated oxidants in addition to alpha-tocopherol (vitamin E) and ascorbic acid (vitamin C), uric acid, glucose and mannitol. [13]

The administration of iron has become accep­ted means of prophylaxis against the develop­ment of iron deficiency in HD patients. [14],[15],[16],[17],[18],[19],[20] However, both iron and erythropoietin (rhEPO) have adverse effects, including potential in­duction of oxidative stress. [21],[22],[23],[24],[25] Several lines of evidence have indicated that the ROS may be involved in the uremic toxicity of patients with end-stage renal disease and decreased anti­oxidant capacity, and concern has been raised about the effects of long-term iron adminis­tration, which is associated with increased plasma lipid peroxidation. [21],[22],[23],[24],[25],[26]

Animal and human data demonstrate bene­ficial effect of N-acetylcysteine (NAC) in models of oxidative stress such as contrast induced acute renal failure [27] and a variety of experimentally or clinically induced ischemia­ reperfusion syndrome of the heart, kidney, lungs and liver. [28],[29] However, its effect in oxi­dative stress induce by intravenous iron the­rapy has not been demonstrated.

We aim in our study determine the oxidative stress and the prophylactic antioxidant effect of the NAC to iron injection in HD patients.


   Material and Methods Top


We studied a total of 14 in- and out-patients on HD program from Nizam's Institute of Medical Sciences (NIMS). The patients in­cluded in the study were more than 18 years of age, undergoing HD with iron deficiency ane­mia. Anemia was defined as blood hemoglobin concentration below 10g/dL. Iron deficiency was defined using National Kidney Founda­tion/DOQI guidelines as having serum ferritin of < 200 ng/dL and/or serum transferrin satu­ration < 20%. The patients excluded from the study were those who had blood transfusion within one month of the study, anemia such that erythrocyte transfusion was imminent, de­monstrated iron overload- serum ferritin > 800 mg/dL, or transferrin saturation > 50%, anemia not caused by iron deficiency, underwent sur­gery or suffered infection within one month of before the start of the study, acute renal fai­lure, hypersensitivity to intravenous iron, and therapy with immunosuppressive agents.

Our study is a prospective, double blinded, randomized, controlled, cross over trial. The patients were randomized into two groups; group 1 received N-acetylcysteine in a dose of 600 mg twice daily for ten days prior to intravenous iron therapy and the other group received a placebo. Both the groups were sub­jected to intravenous iron therapy, 100 mg of iron sucrose in 100 mL of normal saline was administered over a period of one hour in the middle of the week dialysis after HD session. In the second phase, the same patients after the allowance of a week of wash out period for the effect of N-acetylcysteine to wane off were subjected to a cross over. Accordingly, the pa­tients who received N-acetylcycsteine in phase 1 were switched to a placebo in phase 2 and vice versa.

Base line laboratory values of hemoglobin, urea, creatinine, creatinine clearance, serum albumin, total proteins, total cholesterol, triglycerides and viral markers were measured iron, ferritin, transferrin saturation, total iron binding capacity (TIBC), malondiaaldehyde (MDA), high sensitivity C-reactive protein (hsCRP), and antioxidant level like total anti oxidant capacity (TAC) were measured before after iron infusion in each patient.

Measurement of MDA, TAC and hsCRP

MDA is a lipid peroxidation product gene­rated in the tissues by free radical injury. The lipid peroxidation products react with thiobar­bituric acid forming a pink colored adduct on boiling, which is measured at 532 nm. [30] The concentration of MDA is read from a standard calibration curve plotted using TEPP (1, 1, 3, 3­tetra ethoxy propane). The results are presen­ted in nano-moles per mL.

TAC in plasma was measured by decolori­zation assay. [31] The pre-formed radical monoca­tion of 2,2-azinobis- (3-ethylenbenzothiozoline­6-sulfonicacid) (ABTS) is generated by oxida­tion of ABTS (Sigma Chemical Co., USA) with potassium persulfate and is reduced in the pre­sence of hydrogen-donating antioxidants. The inhibition percentage of the ABTS radical cation formation by the added serum sample at a fixed time point is quantified as the result. Trolax (6-hydroxy-2, 5, 7, 8-tetramethychroman­2-carboixylic acid, Aldrich Chemical Co, UK) a water-soluble vitamin-E analogue is used as a standard. The antioxidant capacity of the serum then is expressed in molar Trolax equi­valents per L.

Polystyrene particle coated with monoclonal antibodies specific to human CRP are aggre­gated when mixed with samples containing CRP. These aggregated scatter a beam of light passed through the sample. The intensity of the scattered light is proportional to the concen­ration of the relevant protein in the sample. The result is evaluated by comparison with a standard of a known concentration. The assigned value of CRP in N Rheumatology standard SL is standardized against the international refe­rence preparation BCR-CRM 470. [32],[33]


   Statistical Analysis Top


The statistical analysis was carried out with Sigma graph pad software, USA Version-4. All the data was presented as mean and stan­dard deviation. Unpaired "t" test and ANOVA were used to compare two and more than two treatment groups, respectively. Paired "t" test was used for comparing post treatment group with a baseline. All the efficacy parameters are presented as a percent change from base line. The negative sign indicates a decrease in the value from baseline. P value of <0.05 was con­sidered statistically significant


   Results Top


There were 13 males and only one female in the study with a mean age of 44 ± 10 years. Subjects treated with intravenous iron with or with out N-acetylcysteine were similar with respect to demographic and clinical characte­ristics [Table 1].
Table 1 :Demographic and clinical characteristics (n=14).

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There was an increase in hemoglobin, ferritin and % saturation in both the NAC and placebo groups. The percentage of the increase in the level of hemoglobin and % saturation was higher in the NAC than the placebo group, but was not statistically significant. There was a fall in the level of iron and TIBC in the NAC group [Table 2].
Table 2 :Mean percentage change of laboratory parameter before and after iron sucrose therapy in two
groups - iron + placebo or N-acetylcysteine (NAC).


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There was no change in the levels of the oxidative stress markers in the patients after administration of placebo; however there was a rise in the oxidative markers levels after parenteral iron administration in this group of patients [Table 3]. However, there was no change of the total antioxidant capacity either with placebo or parenteral iron therapy.
Table 3 :Placebo premedicated group: Means of the levels of the oxidative stress parameters.

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Among the oxidative stress markers, MDA demonstrated a statistically significant decrease after NAC and parenteral iron therapy [Table 4]. Though HsCRP showed a declining trend in its levels, which was not statistically signifi­cant, after the administration of NAC and a marginal elevation after parenteral iron com­pared to a placebo. Similarly, the TAC did not change significantly during both phases with and without injection of iron [Table 5].
Table 4 :N-Acetyl cysteine (NAC) premedicated group: Means of the levels of the oxidative stress
parameters.


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Table 5 :Percentage change in biochemical parameters in placebo vs N-acetylcysteine (NAC).

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   Discussion Top


In our study, iron and transferrin saturation levels were measured along with the oxidative stress markers 15 minutes after iron sucrose administration. There was increase in trans­ferrin saturation and a decrease in the iron levels in the placebo and the NAC groups. These data support the notion that oxidative stress occur very early after iron sucrose admi­nistration, at the time point when substantial transferrin is available to bind to free iron. Moreover, there was an increase in the oxi­dative stress markers MDA and HsCRP after parenteral iron sucrose administration both in the placebo and the NAC groups. NAC had reduced the baseline acute systemic generation of oxidative stress when compared to placebo, which was statistically significant with MDA but not with HsCRP though there was a de­clining trend.

In several previous studies, [21],[22],[34],[35] uremic patients on hemodialysis were found to be ex­posed to high levels of ROS. Several studies have demonstrated that intravenous adminis­tration of iron sucrose in dialysis patients re­sults in an increase in markers of lipid per­ oxidation [36],[37] and advanced oxidative protein products. [38],[39] Paik-Seong Lim [40] suggested that the elevated baseline ferritin levels and intra­venous iron infusion exaggerate the increased oxidative stress in uremic patients on HD. Consistent with this observation, the HD pa­tients in our study revealed a significant in­crease in lipid peroxidation marker MDA and decrease in total antioxidant capacity.

An imbalance between the generation and re­moval of ROS and free radicals may be a con­tributory factor for the HD-related complica­tions. Several lines of evidence have indicated that the ROS may be involved in the uremic toxicity of patients with end-stage renal di­sease, and concern has been raised about the effects of long-term iron administration, which is associated with increased plasma lipid per­oxidation.

Animal and human data demonstrate bene­ficial effect of N-acetylcysteine (NAC) in mo­dels of oxidative stress such as contrast in­duced acute renal failure. There are only two studies so far, which tried to know the bene­ficial effect of NAC in the oxidative stress induced by parenteral iron therapy in HD pa­tients. [41],[42]

The time course of transferrin saturation has been reported by Parkkinen et al [43] In HD pa­tients. They reported that transferrin saturation was between 80% and 90% at 3˝ hours fo­llowing iron sucrose administration, and it re­turned to normal in 48 hours. Free iron was not increased unless transferrin saturation was 80% or more. Herrera et al [44] measured plasma MDA levels in HD patients 1 hour following iron sucrose administration and were signifi­cantly increased. Roob et al [45] found plasma MDA levels to peak within 30 minutes after injection of iron sucrose, and Tovbin et al [38] found advanced protein oxidative products to be increased within 3-5 minutes after the ad­ministration of iron sucrose.

Agarwal et al [41] demonstrated that there was no further increase in oxidative stress or renal damage beyond the first 30 minutes of in­jection of iron, despite progressive uptake of iron by transferrin. The results of the antioxi­dant (NAC) administration was mixed, where­as NAC reduced acute generation of oxidative stress and delayed renal generation of oxi­dative stress, there was no improvement in either proteinuria or enzymuria. Another pilot study has demonstrated that intravenous ferric gluconate infusion resulted in a marked in­crease in transferrin saturation and a signi­ficant increase in plasma MDA levels. Urinary MDA levels also increased at the higher dose of iron. There was no evidence of acute renal injury as assessed by albuminuria, proteinuria, and enzymuria. It seems that pre-treatment with NAC had no effect on oxidative stress or the above urinary parameters. [42]

We conclude that the HD patients in our study displayed an increase in the MDA and hsCRP levels after parenteral iron sucrose ad­ministration in both the placebo and the NAC groups. NAC reduced the baseline acute sys­temic generation of oxidative stress when compared to placebo and this reduced oxi­dative stress was maintained even after admi­nistration of intravenous iron therapy.

 
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Correspondence Address:
G Swarnalatha
Department of Nephrology, Nizams Institute of Medical Sciences, Hyderabad
India
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    Tables

  [Table 1], [Table 2], [Table 3], [Table 4], [Table 5]

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    Abstract
    Introduction
    Material and Methods
    Statistical Analysis
    Results
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    References
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