Saudi Journal of Kidney Diseases and Transplantation

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 29  |  Issue : 5  |  Page : 1042--1049

Interleukin-6 gene polymorphisms and serum erythropoietin and hemoglobin in hemodialysis Iraqi patients


Mohanad Yasir Al-Radeef1, Ali Abdulmajid Dyab Allawi2, Hayder Adnan Fawzi3 
1 Department of Clinical Pharmacy, College of Pharmacy, Tikrit University, Tikrit, Iraq
2 Department of Internal Medicine, Baghdad College of Medicine, Baghdad University, Baghdad, Iraq
3 Department of Clinical Pharmacy, Baghdad Teaching Hospital, Baghdad, Iraq

Correspondence Address:
Dr. Mohanad Yasir Al-Radeef
Department of Clinical Pharmacy, College of Pharmacy, Tikrit University, Tikrit
Iraq

Chronic kidney disease (CKD) is characterized by elevated levels of pro-inflammatory cytokines. Interleukin-6 (IL-6) is a pleiotropic and pro-inflammatory cytokine involved in different biological activities such as hematopoiesis, inflammation, and acute-phase response. The rate of IL-6 synthesis and degradation is affected by single nucleotide polymorphisms. This study aimed to evaluate the frequencies of 174G/C IL-6 gene promoter polymorphism in Iraqi hemodialysis (HD) patient and to examine the association between the allelic variations and serum erythropoietin (EPO) and hemoglobin (Hb) levels. The frequencies of IL-6 gene polymorphism were studied in 70 chronic renal failure patients on maintenance HD (patients group) and in 20 healthy participants (control group). Genotyping of IL-6 gene was performed by conventional polymerase chain reaction-restriction fragment length polymorphism. The distribution of IL-6 genotypes between groups was similar, and GG genotype is the most frequent followed by CG and CC genotypes. Control group had a nonsignificant difference in serum EPO levels among different IL-6 genotypes, while patients with GG genotype displayed significant elevation in serum EPO with time, followed by CG and CC genotypes. No significant differences in Hb levels were observed in patients and control groups. A significant positive correlation was observed between serum EPO and Hb in control group with different IL-6 genotypes, while a nonsignificant negative correlation was observed in patients group throughout the study. CKD did not significantly alter IL-6 genotypes, and IL-6 gene polymorphism had a significant effect on serum EPO levels and a nonsignificant effect on Hb levels.


How to cite this article:
Al-Radeef MY, Allawi AA, Fawzi HA. Interleukin-6 gene polymorphisms and serum erythropoietin and hemoglobin in hemodialysis Iraqi patients.Saudi J Kidney Dis Transpl 2018;29:1042-1049


How to cite this URL:
Al-Radeef MY, Allawi AA, Fawzi HA. Interleukin-6 gene polymorphisms and serum erythropoietin and hemoglobin in hemodialysis Iraqi patients. Saudi J Kidney Dis Transpl [serial online] 2018 [cited 2020 Jan 28 ];29:1042-1049
Available from: http://www.sjkdt.org/article.asp?issn=1319-2442;year=2018;volume=29;issue=5;spage=1042;epage=1049;aulast=Al-Radeef;type=0