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| Year : 2010 | Volume
: 21
| Issue : 2 | Page : 269-275 |
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| IgA nephropathy: A clinicopathologic study from two centers in Saudi Arabia |
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Azhar Qayyum Khawajah1, Jaudah Al-Maghrabi2, Hassan D Kanaan3, Saeed Al-Ghamdi4
1 Department of Pathology, College of Medicine, King Abdul Aziz University Hospital; Jeddah, Saudi Arabia
2 Department of Pathology, College of Medicine, King Abdul Aziz University Hospital; Jeddah; Department of Histopathology, King Faisal Specialist Hospital and Research Centre, Jeddah, Saudi Arabia
3 Department of Medicine (Nephrology), King Faisal Specialist Hospital and Research Centre, Jeddah, Saudi Arabia
4 Department of Medicine (Nephrology), King Faisal Specialist Hospital and Research Centre, Saudi Arabia
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| Date of Web Publication | 9-Mar-2010 |
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 Abstract | | |
A total of 42 patients, who were diagnosed to have primary Immunoglobulin A nephropathy (IgAN) at the King Abdul Aziz University Hospital and King Faisal Hospital, Jeddah over the last seven years, were studied. The objective was to analyze their clinical and pathological features and to classify them according to Hass Classification by using light, immunofluorescence and electron microscopy. Majority of the study cases were males in the second, third and fourth decades of life. Hematuria was the most common clinical complaint followed by proteinuria. There were varying degrees of mesangial proliferation. Majority of the cases presented with class-2 followed by class-3. Immunofluorescence demonstrated diffuse granular deposition of IgA in the glomerular mesangium in majority of the cases. Ultrastructural analysis showed electron dense deposits within the matrix of the mesangium and paramesangium in majority of the cases. Sub-endothelial deposits and mesangial interposition were demonstrated in few cases. Extensive effacement with fusion of the visceral epithelial foot processes was detected in only few patients while focal effacement was demonstrated in many cases. Irregularities of the glomerular basement membrane were seen in some cases. We conclude that IgA nephropathy is an immune-complex glomerular disease, which occurs at all ages and with higher frequency in males and presents mostly with hematuria and proteinuria. Public health awareness is seriously needed to perform the investigations at an early stage.
How to cite this article:
Khawajah AQ, Al-Maghrabi J, Kanaan HD, Al-Ghamdi S. IgA nephropathy: A clinicopathologic study from two centers in Saudi Arabia. Saudi J Kidney Dis Transpl 2010;21:269-75 |
How to cite this URL:
Khawajah AQ, Al-Maghrabi J, Kanaan HD, Al-Ghamdi S. IgA nephropathy: A clinicopathologic study from two centers in Saudi Arabia. Saudi J Kidney Dis Transpl [serial online] 2010 [cited 2023 Feb 4];21:269-75. Available from: https://www.sjkdt.org/text.asp?2010/21/2/269/60193 |
| Introduction | |  |
Immunoglobulin A nephropathy (IgAN) is the most frequent primary glomerulonephritis that occurs globally and is recognized as a major cause of end-stage renal disease (ESRD) worldwide. [1],[2],[3] It tends to progress slowly, with progression to ESRD within 25 years in about half of the affected patients. [4],[5],[6],[7],[8] Since 1968, when IgA nephropathy was first described, [9] great strides have been made in clarifying this disease as it poses many challenges to investigators and physicians in its etiology, pathogenesis, prevention, and treatment. [10] The disease presents mainly with hematuria and often proteinuria. [11],[12] Morphologically, it is characterized by diffuse deposits of IgA in the glomerular mesangium and by varying degrees of damage to the glomerular capillary network. [13] Renal biopsy and immunochemical analysis of renal tissue remain the gold standard for diagnosing IgA nephropathy. [14],[15] In the present study, we analyzed the cases of Ig-AN who presented in the last seven years in two hospitals of Jeddah region, with regard to their clinical, light microscopic, immunofluorescence (IF) and electron microscopic features and compared our findings with already published researches. Both these hospitals are tertiary referral centers in the Jeddah region in the western part of Saudi Arabia. Although this study gives reasonable idea about the frequency of IgAN cases in our community, it cannot give an exact prevalence rate, for which we suggest a comprehensive multi-center study.
| Material and Methods | | |
In the last seven years, a total of 42 patients were diagnosed to have primary IgAN on the basis of kidney biopsies performed at the King Abdul Aziz University Hospital and King Faisal Hospital, Jeddah, Saudi Arabia. The diagnosis of IgAN was based on IF microscopy showing mesangial deposits of IgA as the predominant or co-dominant immunoglobulin. HenochSchonlein purpura, systemic lupus erythematosus and liver cirrhosis were excluded by detailed clinical history, physical examination and laboratory findings. Blood and urinary laboratory tests were performed in all patients' including serum creatinine, blood urea nitrogen, urine protein, and urinalysis. Main clinical presentation(s) at the time of performing the renal biopsies were also noted.
For light microscopy, the specimens were fixed in 10% buffered formalin, later dehydrated in ascending grades of series of ethanol, cleared in xylene and embedded in paraffin. Sections were cut at a thickness of three microns, with a standard microtome, mounted on glass slides and stained with hematoxylin and eosin (H& E), periodic acid schiff (PAS), Masson's trichrome (MT) and Jones Methenamine Silver (JMS) stains. Only biopsies containing five or more glomeruli were considered adequate. The lesions were classified according to Hass Classification as follows: [13],[16]
Class-1: Minimal or no mesangial hypercellularity
Class-2: Focal and segmental glomerulosclerosis without cellular proliferation
Class-3: Focal proliferative glomerulonephritis
Class-4: Diffuse proliferative glomerulonephritis
Class-5: ≥ 40% global glomerulosclerosis, and/ or ≥ 40% cortical tubular atrophy
For IF, the fresh tissue in transport media was frozen and cryostat sections were cut at 5µ and incubated with antibodies specific for IgA, IgG, IgM, C3, C1q and fibrinogen. The intensity of immunofluorescence was graded as 0, 1+, 2+, and 3+.
For electron microscopy, small tissue sections (2 × 3 mm) from biopsy cores were fixed in phosphate-buffered 1% gluteraldehyde plus 4% formaldehyde (or 2.5% gluteraldehyde), postfixed in osmium and embedded in plastic. Survey sections were stained with toludine blue and examined to advantage by light microscopy. Thin sections were stained with uranyl acetate and lead citrate for electron microscopic evaluation.
| Results | | |
Of the 42 patients studied, there were 31 males and 11 females with age ranging from six to 72 years [Table 1]. Among males, most of the patients (16.7%) presented in the age-group between 11 and 20 years. The second most common group was between 21 and 30 years and 31 to 40 years, each occurring in 14.3% of the patients. Among females, most of the cases presented in two age-groups, one between 11 and 20 years and the other between 31 and 40 years (7.1% each). Only one female patient presented at 72 years of age.
The clinical characteristics at the time of performing the renal biopsies are shown in [Table 2]. Hematuria was the most common presentation followed by proteinuria. Most of the patients presented with macroscopic hematuria. One patient had severe loin pain along with gross hematuria. A total of 16.7% of the patients had the nephrotic-nephritic syndrome (NNiS) and 21.4% patients had the nephritic syndrome (NiS), indicating that hematuria is a common finding in IgA nephropathy. No case of microscopic hematuria without the NiS was noted. Majority of the patients presented with generalized edema. Twelve cases of the nephrotic syndrome were seen, two of whom were steroid resistant. Three cases presented with acute renal failure; one of them had a lymphoproliferative disorder. Nonspecific presentation was seen in four patients (9.6%) who presented with hepatosplenomegaly, sore throat and/or hypertension.
There were varying degrees of mesangial proliferation and abnormality. The distribution of the glomerular classes of the 42 patients is shown in [Table 3]. The majority of cases had class-2 (45.2%) followed by class-3 (21.4%). Only one case of minimal change disease was seen. Immunofluorescence findings are described in [Table 4]. Two cases could not be processed for IF study. In all cases, IF demonstrated diffuse granular deposits of IgA in the glomerular mesangium. Out of the 40 cases, 24 showed very strong positivity for IgA while only four cases showed a weak positivity for IgA. A total of 22.5% of the biopsies showed IgA and C3 positivity. Two cases with full-house IF and two cases (5%) with C1q, C3 and IgM showed IgA predominance. Electron microscopic (EM) findings of the renal biopsy are shown in [Table 5]. There were electron dense deposits within the matrix of the mesangium and paramesangium in majority of the cases. Sub-endothelial deposits and mesangial interposition were demonstrated in few cases. Only one case showed mesangial, paramesangial, intra-membranous and sub-epithelial deposits. Extensive effacement with fusion of the visceral epithelial foot processes was detected in two patients while focal effacement of the foot processes was demonstrated in 14 patients.
| Discussion | | |
The male preponderance (male to female ratio of 2.8:1) shown in our study is also borne in international literature. [17],[18],[19] In Saudi Arabia, due to cultural reasons women tend to avoid such investigations. But in this context, even in other countries, it has to be remembered that usually more men undergo renal biopsies than women. [14] In one study the research showed the male to female ratio as 58:22. [15]
The worldwide frequency of IgA nephropathy varies widely from 2% to 52% of all renal biopsies. [16] The frequency of 4.7%, noted in our study comes in the lower range, may be due to the lesser number of patients coming for their medical check up. As the awareness and opportunity of medical facilities increase, the frequency of such diseases usually rises. For example, the available evidence shows an increasing incidence in India. In 1987, a frequency of 4.2% was reported from Tamil Nadu, a South Indian state. [20] In 1992, a frequency of 7.24% was reported from New Delhi in North India, [21] and in 1995, a frequency of 10.37% was reported from the North Indian Union Territory of Chandigarh. [22] Saudi Arabia is a rapidly developing country, not only in materialistic terms but also in medical awareness. Thus, with time we expect that in new researches we may see more number of cases of IgA nephropathy. This argument gets more impetus from the other papers published before from Saudi Arabia. In a study carried out in the King Fahad Hospital, [23] IgA nephropathy was seen in 2.8% of their cases while in the King Khalid University Hospital, Riyadh, 3.0% of the cases had IgA nephropathy. [24] The low prevalence in Saudi Arabia, in contrast to the findings of about 40% prevalence in Japan, [25],[26] is quite striking. This difference may be also due to early screening of school children in Japan apart from racial, genetic and environmental factors. One accepted fact is that there is a definite geographic variation. In Europe, the highest frequency was reported from Italy (35.9%). [27] Among Asian countries, it ranged from 25% to 52%, [28] with the highest reported prevalence being from Singapore (52%). [29]
In a study from South Africa, the authors reported a very low prevalence in the black population (0.7%) as compared with Indian residents (13.3%) supporting the conclusion from Yoshino's study that race and ethnicity are important factors affecting the distribution of IgAN. [30] The low frequency of IgAN seen in our study may be due to avoidance of biopsy procedure by the patients. Biopsy policy is certainly an important factor affecting the reported frequency of IgA nephropathy as has been pointed out in a study from the UK in which the frequency varied from 7.1% (1972-78) to 21.1% (1979-86). [31] The biopsy policy varied depending on the nephrologists and the availability of IF facility.
In our study, mesangial proliferation (class-2) was the most common class of IgA nephropathy. Class-3 was the second most common. However, reports from different parts of the world indicate differences in the pattern of disease class. In a recent review, Tumlin et al reported that proliferative and crescentic forms (class-4) were responsible for up to 30% of reported IgA nephropathy. [32]
Since the features of IgA nephropathy identified by light microscopy are nonspecific, IF or immunoperoxidase studies demonstrating a predominant deposition of IgA are essential to establish a definitive diagnosis of IgA nephropathy. The immune-complex deposits are found predominantly within mesangial regions of glomeruli with focal paramesangial or sub-endothelial extension. In our study, IgA was found to be the dominant immunoglobulin in 60% of the cases which is in concordance with the other researches. [26],[28] As reported in literature, [17],[18] a variety of other immunoglobulins and complement are frequently co-distributed with IgA. In our study also, IgA was found to be positive in some cases with other immunoglobulins including IgM and IgG.
In addition to the glomerular alterations, a variety of tubulointerstitial and vascular changes may be identified in patients with IgA nephropathy, including interstitial fibrosis, tubular atrophy, interstitial inflammation, vascular sclerosis, or red-cell casts and proteinaceous casts within the tubules. [33],[34] Also, in our study, we found tubular atrophy in 14.3% of the cases along with interstitial inflammation and fibrosis.
Clinically, in the early stages of the disease, many patients have no obvious symptoms and are unaware of any problems. In these patients, IgA nephropathy may be suspected only during routine screening or investigation of another condition. In our study, only one such case was reported. Most of our patients presented in later stages. Clinical experience tells us that this is not only specific for IgA nephropathy but it is one of the general trends of our society.
Patients with IgA nephropathy usually present with one of the following: [11],[20] episodes of macroscopic hematuria (tea-colored urine) that may coincide with an infection of the upper respiratory tract or abnormal sediment in the urine and proteinuria in patients without symptoms. In our study, hematuria and proteinuria were the most common presentations as mentioned in other studies. [20],[25]
We conclude that IgA nephropathy, the most common form of primary glomerular disease, is an immune-complex-mediated glomerulonephritis defined immunohistologically by the presence of glomerular IgA deposits accompanied by a variety of lesions identified histopathologically. A definitive diagnosis of IgA nephropathy can be made only by renal biopsy and immunohistologic examination. It occurs at all ages, particularly in the second, third and fourth decades and more frequently in males. The disease may show regional variations in frequency which may be explained by genetic differences, differences in renal-biopsy practices or differences in public awareness. The two most common clinical presentations are episodic hematuria and proteinuria. We advise that health authorities should actively participate in public health awareness programs so that the patients may be able to undergo special investigations at an early stage.
| References | | |
| 1. | Tomino Y. IgA nephropathy today. Contrib Nephrol 2007;157:1-255.  [PUBMED] |
| 2. | Donadio JV, Grande JP. IgA nephropathy. N Engl J Med 2002;347:738-48. [PUBMED] |
| 3. | Glassock RJ. Concluding remarks. IgA nephropathy today. Contrib Nephrol 2002;157:169-73. |
| 4. | Coppo R, D'Amico G. Factors predicting progression of IgA nephropathies. J Nephrol 2005; 18:503-12. [PUBMED] |
| 5. | Donadio JV, Bergstralh EJ, Grande JP, Rademcher DM. Proteinuria patterns and their association with subsequent end-stage renal disease in IgA nephropathy. Nephrol Dial Transplant 2002;17:1197-203. |
| 6. | Bartosik LP, Lajole G, Sugar L, Cattran D. Predicting progression in IgA nephropathy. Am J Kidney Dis 2001;58:551-3. |
| 7. | Rauta V, Finne P, Fagerudd J. Factors associated with progression of IgA nephropathy are related to renal function: A model for estimating risk of progression in mild disease. Clin Nephrol 2002; 58:85-94. |
| 8. | Komatsu H, Fujimoto S, Sato Y. "Point of no return (PNR)" in progressive IgA nephropathy: Significance of blood pressure and proteinuria management up to PNR". J Nephrol 2005;18:690-5. |
| 9. | Berger J, Hinglais N. Intercapillary deposits of IgA-IgG. J Urol Nephrol (Paris) 1968;74:694-700. [PUBMED] |
| 10. | Barratt J, Feehally J, Smith AC. The pathogenesis of IgA nephropathy. Semin Nephrol 2004;24:197-217. [PUBMED] |
| 11. | Philibert D. Cattran D. Cook T. Clinicopathologic correlation in IgA nephropathy. Semin Nephrol 2008;28:10-7. |
| 12. | Haubitz M, Wittke S, Weissinger E. Urine protein can serve as a diagnostic tool in patients with IgA nephropathy. Kidney Int 2005;67: 2313-20. |
| 13. | Diagnostic Atlas of Renal Pathology, Glomerular diseases. 107-119. |
| 14. | Panichi V, Pasquariello A, Innocenti M, et al. The Pisa experience of renal biopsies, 19772005. J Nephrol 2007;20:329-35. [PUBMED] |
| 15. | Cook T. Interpretation of renal biopsies in IgA nephropathy. Contrib Nephrol 2007;157:44-9. |
| 16. | Hass M. Histological sub classification of IgA nephropathy: A clinicopathological study of 244 cases. Am J Kidney Dis 1997;29:829-42. |
| 17. | Milovanceva PM. Grcevska L. Dzikova S. Ristovska V. Nikolov V. IgA nephropathy: 23 years of follow-up. Prilozi 2006;27:13-27. |
| 18. | Matsukura H, Miya K, Arai M, Miyawaki T, Inaba S. Minimal change variants with mesangial IgA deposits. Clin Nephrol 2007;68:337-8. [PUBMED] |
| 19. | Westhoff TH, Waldherr R, Loddenkemper C, Ries W, Zidek W, van der Giet M. Mesangial IgA deposition in minimal change nephrotic syndrome: Coincidence of different entities or variant of minimal change disease? Clin Nephrol 2006;65:203-7. [PUBMED] |
| 20. | Date A, Raghvan R, John TJ, Richard J, Kirubakaran MG, Shastry JC. Renal disease in adult Indians: A clinicopathological study of 2827 patients. Q J Med 1987;64:729-37. |
| 21. | Bhuyan UN, Dash SC, Srivasthava RN, Tiwari SC, Malhotra KK. IgA associated glomerulonephritis. J Assoc Physicians India 1992;40: 310-3. |
| 22. | Sehgal S, Datta BN, Sakhuja V, Chugh KS. Primary IgA nephropathy: A preliminary report. Indian J Pathol Microbiol 1995;38:233-7. [PUBMED]  |
| 23. | Lubna AM, John AK, Ramprasad KS, Shaheen FA. Percutaneous renal biopsy and its findings in children and adolescents in Saudi Arabia: A single center experience. Saudi J Kidney Dis Transpl 1997;8(3):289-93. |
| 24. | Abdurrahman MB. Percutaneous renal biopsy in a developing country: Experience with 300 cases. Ann Trop Paeditr 1984;4:25. |
| 25. | Kitajima T, Murakami M, Sakai O. Clinicopathological features in Japanese patients with IgA nephropathy. Jpn J Med 1983;22:219-22. [PUBMED] |
| 26. | Koyama A, Igarashi M, Kobayashi M. Natural history and risk factors of immunoglobulin A nephropathy in Japan. Am J Kidney Dis 1997; 29:526-32. [PUBMED] |
| 27. | Schena FP. Survey of the Italian Registry of Renal Biopsies: Frequency of the renal diseases for 7 consecutive years: The Italian Group of Renal Immunopathology. Nephrol Dial Transplant 1997;12:418-26. [PUBMED] |
| 28. | Muzaffar S, Azad NS, Kayani NS, Pervaz A, Ahmed SH. The frequency of IgA Nephropathy at a single center in Pakistan. JPMA 2003;53: 301-5. |
| 29. | Woo KT, Edmonson RP, Wu AY, Chiang GS, Pwee HS, Lim CH. The natural history of IgA nephritis in Singapore. Clin Nephrol 1986;25: 15-21. |
| 30. | Yoshino NH, Eloisa FF, Glenn MC, Jean LO. Race/ethnicity and disease severity of IgA nephropathy. BMC Nephrol 2004;5:10. |
| 31. | Ballardie FW, O'Donoghue DJ, Feehally J. Increasing frequency of adult IgA nephropathy in UK? Lancet 1987;2:1205. [PUBMED] |
| 32. | Tumlin JA, Madaio MP, Hennigar R. Idiopathic IgA nephropathy: Pathogenesis, histopathology and therapeutic options. Clin J Am Soc Nephrol 2007;2:1054-61. [PUBMED] |
| 33. | Radford MG Jr, Donadio JV Jr, Bergstralh EJ, Grande JP. Predicting renal outcome in IgA nephropathy. J Am Soc Nephrol 1997;8:199-207. [PUBMED] |
| 34. | Fofi C, Pecci G, Galliani M, et al. IgA nephropathy: Multivariate statistical analysis aimed at predicting outcome. J Nephrol 2001;14:280-5. [PUBMED] |
Correspondence Address:
Azhar Qayyum Khawajah
Department of Pathology, College of Medicine, King Abdul Aziz University Hospital, P.O. Box 80205, Jeddah 21589
Saudi Arabia
 Source of Support: None, Conflict of Interest: None  | Check |
PMID: 20228512 
[Table 1], [Table 2], [Table 3], [Table 4], [Table 5] |
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