|Year : 2016 | Volume
| Issue : 3 | Page : 500-506
|Prevalence of asymptomatic urinary abnormalities among adolescents
Mohamed Fouad1, Maher Boraie2
1 Department of Internal Medicine, Nephrology Division, Zagazig University Hospital, Egypt
2 Department of Clinical Pathology, Zagazig University Hospital, Zagazig, Egypt
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|Date of Web Publication||13-May-2016|
| Abstract|| |
To determine the prevalence of asymptomatic urinary abnormalities in adolescents, first morning clean mid-stream urine specimens were obtained from 2500 individuals and examined by dipstick and light microscopy. Adolescents with abnormal screening results were reexamined after two weeks and those who had abnormal results twice were subjected to systemic clinical examination and further clinical and laboratory investigations. Eight hundred and three (32.1%) individuals had urinary abnormalities at the first screening, which significantly decreased to 345 (13.8%) at the second screening, (P <0.001). Hematuria was the most common urinary abnormalities detected in 245 (9.8%) adolescents who had persistent urine abnormalities; 228 (9.1%) individuals had non glomerular hematuria. The hematuria was isolated in 150 (6%) individuals, combined with leukocyturia in 83 (3.3%) individuals, and combined with proteinuria in 12 (0.5%) individuals. Leukocyturia was detected in 150 (6%) of all studied adolescents; it was isolated in 39 (1.6%) individuals and combined with proteinuria in 28 (1.1%) of them. Asymp- tomatic bacteriuria was detected in 23 (0.9%) of all studied adolescents; all the cases were females. Proteinuria was detected in 65 (2.6%) of all the studied adolescents; 45 (1.8%) indivi- duals had <0.5 g/day and twenty (0.8%) individuals had 0.5-3 g/day. Asymptomatic urinary abnormalities were more common in males than females and adolescents from rural than urban areas (P <0.01) and (P <0.001), respectively. The present study found a high prevalence of asymptomatic urinary abnormalities among adolescents in our population.
|How to cite this article:|
Fouad M, Boraie M. Prevalence of asymptomatic urinary abnormalities among adolescents. Saudi J Kidney Dis Transpl 2016;27:500-6
| Introduction|| |
In 2002, the Kidney Disease Outcomes Quality Initiative of the National Kidney Foun- dation defined and classified chronic kidney diseases (CKD).The definition and classifi- cation of CKD were accepted by the Interna- tional Board of Directors of Kidney Disease: Improving Global Outcomes.CKD was de- fined in five stages based on the appearance of proteinuria and glomerular filtration rate le- vels. Patients with early stage CKD had no symptoms, and the majority of individuals in early stage CKD were undiagnosed, even in developed countries.,To reduce the number of patients with both end-stage renal disease (ESRD) and cardiovascular disease,,effective screening and treatment methods for CKD should be established.,,
Most primary chronic glomerulonephritis manifest as asymptomatic proteinuria and/or hematuria.,,,Urinalysis, a simple and inex- pensive test, remains a cornerstone in the eva- luation of the kidney functionand it is help- ful in early detection of renal and urinary abnormalities at relatively low cost.Mass urinary screening helps to determine the preva- lence of renal diseasesand to improve the outcome in the population.
Microscopic hematuria is common in asymp- tomatic adults;the morphology of the red cells and color of the urine may help to iden- tify the origin of bleeding.Asymptomatic persistent proteinuria may be the first mani- festation of significant renal or systemic disease.,,,
The aim of this study was to estimate such prevalence and to set up screening program for early diagnoses and prevention of CKD.
| Patients and Methods|| |
This cross-section study was carried out on adolescent individuals living in Sharkia gover- norate that is located in the East of the Egypt- Delta from December 2012 to October 2014. The initial number of the screened individuals was 3157 adolescent; 657 were excluded from the study, 625 did not deliver urine samples correctly, and 32 were symptomatic. The final included number was 2500 adolescents. The age of the participants in the study ranged from 12 to 15 years (mean age 13.92 ± 0.83). Of the 2500 adolescents (1733 were males and 767 females, ratio 2.25:1), 1750 individuals were from rural and 750 from urban areas (2.3:1). All studied males were circumcised and all the females were sexually inactive, and none of them had any clinical evidence of renal or systemic diseases. The study was approved by the local Institutional Ethics Committee. The aim of the study was explained to the parti- cipants and informed consent was obtained from their parents and school managers.
The participants were instructed how to obtain a clean mid-stream first morning urine specimen to exclude orthostatic proteinuria. The urine containers were brought to the school in the early morning. All specimens were examined by dipstick and light micros- copy.
The dipstick consisted of 10 reagents: ph, specific gravity, protein, blood, glucose, leuko- cytes, nitrites, urobilinogen, bilirubin, and ketones (urine quick test; Combur-10-Test™, Roche, Mannheim, Germany).
Urinalysis was considered abnormal if any the following was detected:
Microscopic analysis was performed by centrifuging 10 mL of well-mixed urine at 1500 g (revolutions per minute) for five mi- nutes in a graduated plastic conical centrifuge tube. Afterward, most of the supernatant was poured off by inversion of the tube, and the sediment was thoroughly resuspended in the remaining supernatant. One drop of this sus- pension was placed on a glass slide, cover- slipped, and examined by subdued bright-field illumination at Xand Xunder a light microscope.
- >5 red blood cell/µL; hematuria (green dots on yellow test: intact erythrocytes or uniform green coloration of test: free hemoglobin or hemolyzed erythrocytes)
- 1+ or greater proteinuria (trace, 1+, 2+, 3+, and 4+ that correspond to 10 mg/dL, 30 mg/dL, 100 mg/dL, 300 mg/dL, and 1000 mg/dL, respectively)
- >5 white blood cell/µL; leukocyturia.
Asymptomatic bacteriuria (ASB) was diag- nosed by the presence of two consecutive clear-voided urine specimens both yielding positive cultures 10colony-forming unit of the same uropathogen.
All adolescents with urinary abnormalities in the first screening were reexamined after two weeks to exclude transient causes of urinary abnormalities, false positive results, and menses in females. Persistently positive individuals were subjected to further analysis; the urine samples of the hematuric group were examined by double phase microscopy to differentiate glomerular (90% of the film dysmorphic) form non glomerular hematuria (90% of the film were isomorphic), while persistently proteinuric individuals were further evaluated by quanti- tative measurements of 24 h proteinuria.
All adolescents with urinary abnormalities in the second screening were subjected to further investigations as indicated: serum creatinine levels, C3 levels, antistreptolysin O titers, and pelvic-abdominal ultrasounds.
| Statistical analysis|| |
Data were collected, entered, and analyzed in an Statistical Package for the Social Sciences (SPSS) version 19.0, (SPSS Inc., Chicago, Ill, USA). Data were expressed as a mean ± standard deviation. z-test, and Chi-square (c2 test were used in the analysis of the data. P <0.05 was considered significant.
| Results|| |
The prevalence of asymptomatic urinary ab- normalities among the studied individuals was 803 (32.1%) in the first screening. Hematuria, proteinuria, and leukocyturia were detected in 660 (26.4%), 95 (3.8%), and 288 (11.5%), res- pectively. In the second screening, asymp- tomatic urinary abnormalities significantly decreased to 345 (13.8%) P <0.001, similarly hematuria and leukocyturia were significantly decreased to 245 (9.8%) P <0.01 and 150 (6%) P = 0.03, respectively, although proteinuria also decreased to 65 (2.6%) but insignificantly [Figure 1] and [Table 1].
|Table 1. The results of the first and second screen and significant difference in between|
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Asymptomatic urinary abnormalities were more common in adolescent males from rural areas [Table 2].
|Table 2. Total urinary abnormalities frequently seen in males gender and rural areas, similarly hematuria more frequent in males and rural areas, proteinuria more frequent only in rural areas lastly leukocyturia more frequent in female gender and rural areas|
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Hematuria was non glomerular and glome- rular in 228 (9.1%) and 17 (0.7%) studied indi- viduals, respectively. Hematuria was isolated, combined with leukocyturia, and combined with proteinuria in 150 (6%), 83 (3.3%), and 12 (0.5%), respectively.
Leukocyturia was detected in 150 (6%), iso- lated in 39 (1.6%) and combined with protei- nuria in 28 (1.1%). ASB was detected in 23 (0.9%) of all adolescents; all cases were females, and Escherichia coli was isolated from all of them.
Proteinuria was <0.5 g/24 h and ranging from 0.5 to 3 g/24 h in 45 (1.8%) and 20 (0.8%) individuals, respectively. Finally, isolated pro- teinuria was presented in 25 (1%) of the studied individuals.
Schistosoma hematobium ova was detected in 208 (8.3%) of all the studied adolescents. Investigations of adolescents with urinary ab- normalities revealed that 12 of them had small renal stones <6 mm, and other six individuals had mild hydronephrosis. There were no cases of poststreptococcal glomerulonephritis and kidney function was within the normal range in all the study individuals.
| Discussion|| |
A major problem in nephrology practice in developing countries is how to define stra- tegies that can detect early urinary abnormality who are at risk of developing CKD later in life; a screening program is recommended in this regard.
In the current study, the prevalence of asymp- tomatic urinary abnormalities in adolescents was 32.1% in the first screening, and these abnormalities persisted only in 13.8% in the second screening. Our findings are in contrast to previous study by Bakr et althat found only 1.3% of the samples studied had urinary abnormalities at the first screening, and these abnormalities persisted in 0.72% at the second screening. This wide difference implied the marked increasing of the annual incidence of asymptomatic urinary abnormalities and high- lighting the magnitude of such problem in Egypt. However, our results were comparable to Bolivian studywhich reported urinary abnormalities in 30.3% at the first screening and persisted in 7.2% at the second screening. Contrary to our results, others studies showed lower prevalence of asymptomatic urinary abnormalities in elementary school children such as 0.62% in Japan,0.3% in Taiwan,1.9% in Malaysia,and 5.25% in Nigeria.All previous screening was in primary school children; all younger than adolescents sharing in the current study that suggest increased prevalence in older age. Accordingly, some countries such as Japan introduced an annual urinalysis screening program for every school child in 1973, for every working adult in 1972, and for every resident older than 40 years of age in 1982.,,In addition, different ethni- cities and socioeconomic factors may play a role.
Hematuria was the most common abnor- mality found in our study with the prevalence of 9.8% of all adolescents. This was compa- rable to other previous studies in Egypt,Bolivia,and Lebanon,but in contrast with to a Nigerian studywhere proteinuria was the most common positive finding. The high pre- valence of hematuria particularly non glo- merular (9.1%) in the current study was attributed to schistosomal infestation (8.3%) in the study patients. Hence, long-term follow up for adolescents with microscopic hematuria is advisable.
]The prevalence of isolated hematuria and of hematuria combined with proteinuria was comparable to the Finnish study which found that the prevalence of a combination of hema- turia and proteinuria was significantly less common than either isolated proteinuria or hematuria (0.7%).Adolescents with such a combination are more likely to have significant renal disease and require further evaluation.
The prevalence of proteinuria in our study was consistent with another study from Egypt (2.13%),Northern Iran (1.6%),Nigeria (3.5%),and India (2.6%);similar socioeco- nomic factors may explain this agreement with our results. This prevalence is higher than that reported from Tokyoand Lebanon(0.08% and 0.1%, respectively).
The levels of proteinuria are one of the strongest predictors for renal function deterio- ration.Asymptomatic proteinuria warrants further work-up to detect and even prevent ESRD.
The prevalence of leukocyturia in our study was comparable to others two studies from Egypt (4.9%)and (7%) and one from Tur- key (4.5 %). Contrary to our results a lower prevalence was reported from Japan (0.29%).The differences in between the previous studies could be explained by the different methods of diagnosis and socio-economic factors.
ASB prevalence in our study was comparable to that in school-age girls (2%).Contrary to our results, a higher prevalence of ASB (5%) was observed in young sexually active women.Hematuria as predominant asymptomatic uri- nary abnormalities was more common in males. This was attributed to males more liable to Schistosoma infestation by swimming in Nile River, especially in rural areas.
Leukocyturia and ASB were more common in females; consistent with other studies from Lebanon,Nigeria,and Vietnam.The female predominance was attributed to the fact that girls have short urethra, which predisposes them to ascending bacterial infection.
In our study, asymptomatic urinary abnor- malities were more prevalent in rural areas than urban, and this may be attributed to poor socioeconomic status, poor health education, and infrequent routine medical visits. Our findings were comparable to those reported from another Egyptian studyand Eastern Turkey study.
We conclude that our present study found a high prevalence of asymptomatic urinary ab- normalities in Egyptian adolescents. We pro- pose accordingly a national screening program for early detection of asymptomatic urinary ab- normalities, which may help reducing the ESRD population in Egypt.
Conflict of interest: No conflict of interest has been declared by the authors.
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Department of Internal Medicine, Nephrology Division, Zagazig University Hospital, Zagazig
[Table 1], [Table 2]
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