Saudi Journal of Kidney Diseases and Transplantation

ORIGINAL ARTICLE
Year
: 2013  |  Volume : 24  |  Issue : 4  |  Page : 751--758

Immuno-histochemistry analysis of Helicobacter pylori antigen in renal biopsy specimens from patients with glomerulonephritis


Qian Li1, Xi Lin2, Zhongbiao Wu2, Lihuan He1, Weijun Wang2, Qizhi Cao1, Jianzhong Zhang1 
1 State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
2 The First People's Hospital of Wenling, the Affiliated Wenling Hospital of Wenzhou Medical College, Zhejiang, China

Correspondence Address:
Jianzhong Zhang
National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Beijing
China

This study was conducted to investigate the relationship between Helicobacter pylori infection and three varieties of glomerulonephritis. Renal biopsy specimens from patients with Henoch Schonlein Purpura nephritis (HSPN; n = 10), membranous nephropathy (MN; n = 9) and lupus nephritis (LN; n = 27) were studied using immuno-histochemical labeling to clarify the etiological significance of H. pylori antigen in this disease. Immuno-histochemical labeling was performed using a mixture of anti-H. pylori-antibody-positive serum from nine volunteers; a mixture of anti-H. pylori-antibody-negative serum from nine volunteers was used as control. Staphylococci protein-A labeled by horseradish peroxidase was used as the second antibody in this study. A total of 34 of the 48 specimens revealed positive reaction with the anti-H. pylori-positive serum and five of the 48 specimens revealed positive reaction with the anti-H. pylori-negative serum. Positive reaction against anti-H. pylori-positive serum was seen in 10/10 patients with HSPN, six of nine patients with MN and 18/27 patients with LN. Statistical analysis showed that the difference of the positive reaction between anti-H. pylori-positive and negative sera was significant (χ 2 = 36.318, P = 0.000). Our study indicates that H. pylori infection may be associated with the development and/or progression of HSPN, MN and LN.


How to cite this article:
Li Q, Lin X, Wu Z, He L, Wang W, Cao Q, Zhang J. Immuno-histochemistry analysis of Helicobacter pylori antigen in renal biopsy specimens from patients with glomerulonephritis.Saudi J Kidney Dis Transpl 2013;24:751-758


How to cite this URL:
Li Q, Lin X, Wu Z, He L, Wang W, Cao Q, Zhang J. Immuno-histochemistry analysis of Helicobacter pylori antigen in renal biopsy specimens from patients with glomerulonephritis. Saudi J Kidney Dis Transpl [serial online] 2013 [cited 2022 Jan 17 ];24:751-758
Available from: https://www.sjkdt.org/article.asp?issn=1319-2442;year=2013;volume=24;issue=4;spage=751;epage=758;aulast=Li;type=0