Saudi Journal of Kidney Diseases and Transplantation

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 31  |  Issue : 5  |  Page : 937--945

Aspirin-Triggered Lipoxin Protects Lipopolysaccharide-Induced Acute Kidney Injury via the TLR4/MyD88/NF-κB Pathway


Pei Zhang1, Hongjun Peng1, Chunlin Gao1, Zhongmin Fan2, Zhengkun Xia1 
1 Department of Pediatrics, Jinling Hospital, Southern Medical University, Nanjing, Jiangsu, China
2 Department of Pediatrics, BenQ Medical Center, Nanjing Medical University, Nanjing, Jiangsu, China

Correspondence Address:
Zhengkun Xia
Department of Pediatrics, Jinling Hospital, Southern Medical University, Nanjing, Jiangsu
China

The protective effect of aspirin-triggered lipoxin (ATL) on lipopolysaccharide (LPS)-induced acute kidney injury (AKI) and its possible mechanisms were explored. To induce acute renal injury, mice were treated with LPS. Concentration of serum creatinine (SCr) and blood urea nitrogen (BUN) was detected, and inflammatory cytokines and AKI biomarkers were determined by ELISA. The relative protein expression levels of TLR4/myeloid differentiation factor 88 (MyD88)/NF-κB signal pathway was assessed by Western blot. Mice subjected to LPS (4 mg/kg) treatment exhibited AKI demonstrated by markedly increased SCr and BUN levels compared with controls (P <0.01). Treatment with ATL decreased SCr and BUN levels after LPS injection (P <0.01). AKI biomarkers, such as urine NGAL, KIM-1, netrin-1, and L-FABP levels, increased by LPS and were inhibited by ATL (P <0.01). ATL also reduced LPS-induced secretion of inflammatory cytokines such as tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6, and IL-8 (P <0.01). Furthermore, mice pretreated with ATL before exposure to LPS showed a reduction in TLR, MyD88, and p65 phosphorylation (P <0.01), which are the key factors of the TLR/MyD88/NF-κB signaling pathway. These results indicated that ATL had protective effects on renal function and showed amelioration of LPS-induced kidney injury. The mechanisms underlying the protective effects of ATL can be considered are related to attenuation of the TLR4/MyD88/NF-κB signaling pathway.


How to cite this article:
Zhang P, Peng H, Gao C, Fan Z, Xia Z. Aspirin-Triggered Lipoxin Protects Lipopolysaccharide-Induced Acute Kidney Injury via the TLR4/MyD88/NF-κB Pathway.Saudi J Kidney Dis Transpl 2020;31:937-945


How to cite this URL:
Zhang P, Peng H, Gao C, Fan Z, Xia Z. Aspirin-Triggered Lipoxin Protects Lipopolysaccharide-Induced Acute Kidney Injury via the TLR4/MyD88/NF-κB Pathway. Saudi J Kidney Dis Transpl [serial online] 2020 [cited 2021 Sep 27 ];31:937-945
Available from: https://www.sjkdt.org/article.asp?issn=1319-2442;year=2020;volume=31;issue=5;spage=937;epage=945;aulast=Zhang;type=0