Saudi Journal of Kidney Diseases and Transplantation

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 31  |  Issue : 5  |  Page : 982--997

A Comparative Study of the Use of Dexamethasone, N-acetyl Cysteine, and Theophylline to Ameliorate Renal Ischemia–Reperfusion Injury in Experimental Rat Models: A Biochemical and Immuno-histochemical Approach


Ahmed Salaheldin Sayed Zeid1, Safinaz Salaheldin Sayed2 
1 Department of Pediatrics, Pediatric Nephrology Unit, Faculty of Medicine, Cairo University, Cairo, Egypt
2 Department of Medical Histology and Cell Biology, Faculty of Medicine, Cairo University, Cairo, Egypt

Correspondence Address:
Ahmed Salaheldin Sayed Zeid
Department of Pediatrics, Pediatric Nephrology Unit, Faculty of Medicine, Cairo University, Cairo 12613
Egypt

Renal ischemia–reperfusion injury (IRI) is commonly encountered in clinical practice during renal transplantation. In a trial to find the drug that best safeguards the kidney against IRI, dexamethasone (Dex), N-acetyl cysteine (NAC), and theophylline (Theo) were tested in experimental rat models. This study included 105 adult male albino rats, which were randomly assigned to the following five groups: Group I – sham-operated, n = 5, Group II – IRI n = 25, Group III – IRI + Dex n = 25, Group IV – IRI + NAC n = 25, and Group V –IRI + Theo n = 25. IRI was induced for 40 min followed by reperfusion. Rats were sacrificed 1, 2, 4, 6, and 24 h after reperfusion. This was preceded by blood and urine sampling for biochemical study of serum Cystatin C (Cys C), serum creatinine, and urinary Cys C. Kidneys were processed for histopathological evaluation and immune-histochemical staining for Cys C. The expression of Cys C in the proximal tubular cells was significantly lower in the IRI group compared to that of the sham group. There was a significant rise in the levels of serum and urinary Cys C after 1 h in the IRI group, while the rise in creatinine occurred later. Dex was superior to NAC and Theo 24 h after the IR insult, and the serum levels of creatinine and Cys C were significantly lower in this group than the other two drug groups (P <0.001 in both cases). Our study revealed a clear benefit for the use of Dex to ameliorate IRI over NAC and Theo if used immediately following the insult. The effect is evident 24-h after its use. The role of serum Cys C as an early marker of acute kidney injury compared to serum creatinine is confirmed.


How to cite this article:
Sayed Zeid AS, Sayed SS. A Comparative Study of the Use of Dexamethasone, N-acetyl Cysteine, and Theophylline to Ameliorate Renal Ischemia–Reperfusion Injury in Experimental Rat Models: A Biochemical and Immuno-histochemical Approach.Saudi J Kidney Dis Transpl 2020;31:982-997


How to cite this URL:
Sayed Zeid AS, Sayed SS. A Comparative Study of the Use of Dexamethasone, N-acetyl Cysteine, and Theophylline to Ameliorate Renal Ischemia–Reperfusion Injury in Experimental Rat Models: A Biochemical and Immuno-histochemical Approach. Saudi J Kidney Dis Transpl [serial online] 2020 [cited 2023 Jan 27 ];31:982-997
Available from: https://www.sjkdt.org/article.asp?issn=1319-2442;year=2020;volume=31;issue=5;spage=982;epage=997;aulast=Sayed;type=0